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roche lightcycler system  (Roche)


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    Structured Review

    Roche roche lightcycler system
    Roche Lightcycler System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/roche+lightcycler+480+system/LightCycler+480+System/pmc13091439-96-6-6
    Average 99 stars, based on 6 article reviews
    roche lightcycler system - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The ANXA2P1-hnRNP F-HK2/c-Myc Positive Feedback Loop Promotes Proliferation and Glycolytic Metabolism in Gastric Cancer.
    Article Snippet: cDNA synthesis was performed from 1 μg of total RNA via the Reverse Transcription Kit (TaKaRa, Japan). .. Quantitative PCR was conducted on a Roche LightCycler 480 system (Roche, Switzerland) via the SYBR Green PCR Kit (Takara, Japan). ..

    Article Title: LOX-A4 shapes Triticum urartu gene pools and contributions to the A subgenome of polyploid wheat.
    Article Snippet: .. The qPCR assays used SYBR Green qPCR Master Mix (Vazyme, Cat# Q711) and were conducted on the Roche LightCycler® 480 System (Roche Applied Science, Indianapolis, IN). ..

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy.
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5mm) per sample (2 × 2min at 25Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5 mm) per sample (2 × 2 min at 25 Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    Article Title: A Self-amplifying mRNA vaccine for infectious laryngotracheitis virus (ILTV) induces efficient protective immunity
    Article Snippet: .. Quantitative PCR was performed on a Roche LightCycler 480 system, and the data were analyzed with the built-in LightCycler® 480 software (version 1.5; Roche Diagnostics, Switzerland). .. Serum concentrations of IFN-γ and IL-2 were quantified according to the manufacturer's protocols utilizing commercial chicken-specific ELISA kits (Mlbio, China).

    SYBR Green Assay:

    Article Title: The ANXA2P1-hnRNP F-HK2/c-Myc Positive Feedback Loop Promotes Proliferation and Glycolytic Metabolism in Gastric Cancer.
    Article Snippet: cDNA synthesis was performed from 1 μg of total RNA via the Reverse Transcription Kit (TaKaRa, Japan). .. Quantitative PCR was conducted on a Roche LightCycler 480 system (Roche, Switzerland) via the SYBR Green PCR Kit (Takara, Japan). ..

    Article Title: LOX-A4 shapes Triticum urartu gene pools and contributions to the A subgenome of polyploid wheat.
    Article Snippet: .. The qPCR assays used SYBR Green qPCR Master Mix (Vazyme, Cat# Q711) and were conducted on the Roche LightCycler® 480 System (Roche Applied Science, Indianapolis, IN). ..

    Article Title: Mechanisms of Gui Zhi Fu Ling Formula in Liver Fibrosis: Metabolomics and Gut Microbiota Analysis
    Article Snippet: .. Amplification products were analyzed using a Roche LightCycler 480 system (Roche, Basel, Switzerland) with the LightCycler 480 SYBR Green I Master Mix (04887352001, Roche, Basel, Switzerland). .. RT‐qPCR was performed on a Thermo Fisher ABI QuantStudio 6 Flex system.

    Article Title: Insects manipulate host nuclear trafficking and epigenetic regulation to facilitate herbivory.
    Article Snippet: For rice samples, treated sheaths were ground in liquid nitrogen, and total RNA was extracted using the TRIzol Total RNA Isolation Kit (#9109, Takara, Dalian, China) according to the manufacturer’s instructions. .. First-strand cDNA was synthesized using the HiScript II Q RT SuperMix (#R212-01, Vazyme, Nanjing, China). qRT-PCR was performed on a Roche LightCycler® 480 system with the SYBR Green Supermix Kit (#11202ES08, Yeasen, Shanghai, China). ..

    Polymerase Chain Reaction:

    Article Title: The ANXA2P1-hnRNP F-HK2/c-Myc Positive Feedback Loop Promotes Proliferation and Glycolytic Metabolism in Gastric Cancer.
    Article Snippet: cDNA synthesis was performed from 1 μg of total RNA via the Reverse Transcription Kit (TaKaRa, Japan). .. Quantitative PCR was conducted on a Roche LightCycler 480 system (Roche, Switzerland) via the SYBR Green PCR Kit (Takara, Japan). ..

    Amplification:

    Article Title: Mechanisms of Gui Zhi Fu Ling Formula in Liver Fibrosis: Metabolomics and Gut Microbiota Analysis
    Article Snippet: .. Amplification products were analyzed using a Roche LightCycler 480 system (Roche, Basel, Switzerland) with the LightCycler 480 SYBR Green I Master Mix (04887352001, Roche, Basel, Switzerland). .. RT‐qPCR was performed on a Thermo Fisher ABI QuantStudio 6 Flex system.

    Purification:

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy.
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5mm) per sample (2 × 2min at 25Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5 mm) per sample (2 × 2 min at 25 Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    Reverse Transcription:

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy.
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5mm) per sample (2 × 2min at 25Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5 mm) per sample (2 × 2 min at 25 Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    cDNA Synthesis:

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy.
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5mm) per sample (2 × 2min at 25Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    Article Title: Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy
    Article Snippet: To isolate RNA from mouse tissues stored in RNAlater (Sigma-Aldrich), samples were first homogenized in the TRIzol reagent (Invitrogen) using TissueLyser II (Qiagen) with one stainless steel bead (5 mm) per sample (2 × 2 min at 25 Hz) and then the manufacturer’s instructions were followed. .. Purified RNA was measured by Nanodrop (Thermo Fisher) and reverse transcription was performed using Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific). qPCR was performed on the Roche Lightcycler 480 system using Blue S’Green qPCR kit (Biozym). ..

    Software:

    Article Title: A Self-amplifying mRNA vaccine for infectious laryngotracheitis virus (ILTV) induces efficient protective immunity
    Article Snippet: .. Quantitative PCR was performed on a Roche LightCycler 480 system, and the data were analyzed with the built-in LightCycler® 480 software (version 1.5; Roche Diagnostics, Switzerland). .. Serum concentrations of IFN-γ and IL-2 were quantified according to the manufacturer's protocols utilizing commercial chicken-specific ELISA kits (Mlbio, China).

    Synthesized:

    Article Title: Insects manipulate host nuclear trafficking and epigenetic regulation to facilitate herbivory.
    Article Snippet: For rice samples, treated sheaths were ground in liquid nitrogen, and total RNA was extracted using the TRIzol Total RNA Isolation Kit (#9109, Takara, Dalian, China) according to the manufacturer’s instructions. .. First-strand cDNA was synthesized using the HiScript II Q RT SuperMix (#R212-01, Vazyme, Nanjing, China). qRT-PCR was performed on a Roche LightCycler® 480 system with the SYBR Green Supermix Kit (#11202ES08, Yeasen, Shanghai, China). ..

    Quantitative RT-PCR:

    Article Title: Insects manipulate host nuclear trafficking and epigenetic regulation to facilitate herbivory.
    Article Snippet: For rice samples, treated sheaths were ground in liquid nitrogen, and total RNA was extracted using the TRIzol Total RNA Isolation Kit (#9109, Takara, Dalian, China) according to the manufacturer’s instructions. .. First-strand cDNA was synthesized using the HiScript II Q RT SuperMix (#R212-01, Vazyme, Nanjing, China). qRT-PCR was performed on a Roche LightCycler® 480 system with the SYBR Green Supermix Kit (#11202ES08, Yeasen, Shanghai, China). ..



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